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. 2018 Feb 1;7(2):bio029637. doi: 10.1242/bio.029637

Fig. 2.

Fig. 2.

HDAC1 is required for Notch target gene expression. (A-D′) RNAi knockdown of HDAC1 with en-Gal4 reduces Cut (A-B′) and Wingless (C-D′) protein levels in the posterior half of the wing disc. GFP marks the expression domain of en-Gal4. (E-E′) Expression pattern of the vg(BE)-lacZ reporter gene in a control wing imaginal disc. (F-F′) Knockdown of HDAC1 by RNAi with hh-Gal4 eliminates vg(BE)-lacZ expression in the posterior compartment of the wing disc. (G-G′) Expression pattern of the E(spl)m8-lacZ reporter gene in a control wing imaginal disc. (H-H′) Knockdown of HDAC1 by RNAi with hh-Gal4 reduces the level of E(spl)m8-lacZ expression in the posterior compartment of the wing disc. Discs in E-H′ were stained with anti-β-Gal and anti-GFP. DNA was stained with DAPI. Posterior cells are marked by GFP. (I-J′) MARCM clones of HDAC1[303] in the wing disc results in a decrease of Cut (I,I′) and Wingless (J,J′) protein levels. (K-L′) Overexpression of HDAC1 in MARCM clones of HDAC1[303] in the wing disc restores Cut (K,K′) and Wingless (L,L′) protein levels. Mutant clones are marked by the presence of GFP. Scale bars: 50 μm.