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. 2018 Feb 15;7(2):bio030494. doi: 10.1242/bio.030494

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© 2018. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 4. — Initiation of claudin 5a expression coincides with blood vessel formation. (A,B) Still images of two time lapses of blood vessel development in Tg(kdrl:mCherry)^is5;TgBAC(cldn5a:EGFP)^vum2 larva at 96 hpf. Images were taken every 2.5 h for a period of 12.5 h on blood vessels in the optic tectum of the midbrain. Images of single channels show blood vessel development (kdrl:mcherryCAAX) on the top row, coinciding with development of claudin 5a in the middle row. Merged images are shown in the bottom row. Scale bars: 20 μm. See corresponding movies of time lapses (Movies 1 and 2).