Fig. 2.

Identification of a functional NLS within mouse Gemin4. (A) Schematic of mouse Gemin4 protein. (B) Top row: full-length (GFP-Gemin4) and an N-terminal (GFP-G4ΔCT) fragment of Gemin4 localize to the nucleoplasm. A C-terminal (GFP-G4ΔNT) fragment accumulates in the cytoplasm. Middle row: expression of internal deletion constructs shows that only NLS2 is required for nuclear accumulation of Gemin4. Bottom row: although GFP alone displays pan-cellular localization, the minimal NLS2 motif (GFP-NLS2 min) is primarily nuclear. The extended NLS sequence (GFP-NLS2ext) is exclusively nuclear. Insets in upper left corners of panels show the DAPI-stained nuclei. Scale bar: 5 µm.