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Quantification of SMN and Gemin2 phenotypes in HeLa cells overexpressing Gemin4. HeLa cells were transfected with either myc- (A-D) or GFP-Gemin4 (E-H) and then co-stained with antibodies targeting SMN or Gemin2, as shown. Each nucleus was scored for the presence or absence of nuclear foci (B,F) as well as for nucleoplasmic accumulation (D,H). Scale bars: 5 µm.
