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. 2017 Dec 30;46(5):2321–2334. doi: 10.1093/nar/gkx1305

Figure 3.

Figure 3.

Spn1 binds nucleosomal arrays. The binding of Spn1 or Spn1141–305 to nucleosomal arrays assembled on DNA containing 12 repeats of the 207 base pair, 601 nucleosome positioning sequence was assessed by AUC and EMSA. Sedimentation velocity curves for (A) wild type Spn1 or (B) Spn1141–305 added to the nucleosomal arrays at a Spn1:nucleosome molar ratio of 0.5 (red circles), 1.0 (blue squares) or 2.0 (green diamonds) in the presence of 2.5 mM NaCl. Orange triangles are the arrays alone, (C) EMSA of wild type Spn1 and Spn1141–305 with 207–12 nucleosomal arrays using 1% agarose gels.