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. 2018 Jan 27;46(5):2660–2677. doi: 10.1093/nar/gky044

Figure 7.

Figure 7.

DNA binding properties of wild-type and mutants PB Cysteine-Rich Domain. (A) Wild type PB CRD (GST-PB(537–594)) formed different types of complexes in the presence of the LE1–35 and Random substrate. Complexes were assembled in the presence of LE1–35-Cy3 or Random-Cy3 substrates (100 nM) and 0.33 μM, 1 or 3 μM concentration of GST-PB(537–594) before analysis on a 5% acrylamide, 0.5× TBE gel. (B) Wild-type or mutants GST-PB(537–594)/LE1–35 complexes were assembled in the presence of 100 nM of LE1–35-Cy3 substrate and 0.03 μM, 0.11, 0.33, 1 or 3 μM of GST fused PB(537–594). Complexes were further analysed by EMSA on a 5% acrylamide, 0.5× TBE gel.