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. 2018 Jan 18;46(5):2417–2431. doi: 10.1093/nar/gkx1318

Figure 3.

Figure 3.

PARP3- and PARP2-catalyzed formation of MAR(PAR)–DNA adducts at the phosphorylated DSB and SSB termini of plasmid-based DNA substrates. (A) Denaturing PAGE analysis of PARP-generated products of ADP-ribosylation of 1 nM 5′-[32P]labeled linearized nicked pML2 plasmid under the standard reaction conditions. (B) ADP-ribosylation of a circular plasmid containing multiple SSBs. Fifty-nanomolar PARPs were incubated with 2 nM pML2 circular plasmid containing a 5′-[32P]labeled 1 nt gap placed upstream of two nicks under the standard reaction conditions.