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. 2018 Jan 18;46(5):2417–2431. doi: 10.1093/nar/gkx1318

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© The Author(s) 2018. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 7. — Differential loss of the PAR signal in dot blotting experiments during successive procedures of gDNA purification from HeLa PARG^KD cells treated with bleomycin as compared to control gDNA mixed with free PAR. Graphical representation of the PAR level detected in 1200-ng dots of gDNA after each purification round. Control gDNA was purified from untreated HeLa BD650 cells. The PAR signal after the first purification round was set to 100%. The error bars represent SD (n = 3). For details, see ‘Materials and Methods’ section and Supplementary Figure S14.