Table 2. Direct cloning of the 14 kb lux gene cluster from diluted P. phosphoreum genomic DNA with ExoCET.
P. phosphoreum (ng) (BamHI + KpnI) | B. subtilis (μg) (BamHI) | Vector | c.f.u. (/ml) | Correct/checked |
---|---|---|---|---|
10 | 10 | p15A-cm | 200 ± 2 | 7/12 |
5 | 10 | p15A-cm | 142 ± 22 | 5/12 |
2 | 10 | p15A-cm | 102 ± 8 | 2/12 |
1 | 10 | p15A-cm | 104 ± 18 | 2/24 |