Figure 1.

A defined site-specific SSB structure can be repaired in the Xenopus HSS system. (A) Schematic diagram of generating SSB and DSB plasmid structures. (B) The defined SSB structure is gradually repaired in HSS system. SSB repair intermediate products from HSS system were isolated at different time points and treated by SbfI, followed by examination via agarose gel electrophoresis. (C) (Top) CTL or SSB plasmid was added to HSS, which was supplemented with [³²P-α]-dATP, for a 30-min incubation. Then NPE was added for continuous incubation for different time as indicated and samples were examined on agarose gel. (Bottom) Quantification of DNA synthesis of CTL or SSB plasmid in the HSS/NPE system shown in the top.