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. 2018 Jan 19;46(5):2479–2494. doi: 10.1093/nar/gky020

Figure 4.

Figure 4.

The SSB-induced ATR DDR pathway is essential for SSB repair. (A) SSB plasmid was added to HSS with or without VE-822 for different time as indicated. SSB repair products were isolated and examined on agarose gel (Ethidium bromide staining). (B) Quantification of SSB repair capacity (circular/(circular + nicked) × 100) with or without VE-822 treatment in the HSS system from (A). (C) SSB plasmid was added to mock- or ATRIP-depleted HSS for different time as indicated. SSB repair products were isolated and examined on agarose (Ethidium bromide staining). (D) Quantification of SSB repair capacity in mock- or ATRIP-depleted HSS system from (C) using a similar method described in (B).