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. 2017 Dec 27;46(5):2600–2612. doi: 10.1093/nar/gkx1287

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© The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 2. — The 5′-UTR of hdeD mRNA is targeted by both CyaR and RprA in vitro. (A) Lead acetate (PbAC) probing of 5′end-radiolabeled hdeD+195 incubated in presence or absence of CyaR and RprA. OH, alkaline ladder; T1, RNase T1 ladder. The numbers to the left indicate sequence positions with respect to the +1 of hdeD. (B) Validated pairing between hdeD and both sRNAs. (C) In-line (MgCl₂) probing of 5′end-radiolabeled hdeD+195 in presence or absence of RprA. Samples were incubated with MgCl₂ during 48 h. OH, alkaline ladder; T1, RNase T1 ladder. (D) Representation of the second validated pairing site of RprA on hdeD mRNA.