Figure 5.

Interchanging the seed sequence of CyaR and RprA also interchanges the ability to induce hdeD mRNA decay. (A) Secondary structures of CyaR and RprA sRNAs determined using Mfold software (35) and visualized with VARNA software (36). To construct CyaR-seed* and RprA-seed*, the hairpins bearing the seed region of CyaR and RprA were switched. Exchanged nucleotides are circled. Binding sites are indicated in black. (B) β-Galactosidase assays with hdeD+65 transcriptional lacZ fusion in a ΔcyaR ΔrprA background. Overexpression of cyaR, cyaR-seed*, rprA or rprA-seed* was induced by addition of 0.1% arabinose when cells reached an OD_{600 nm} of 0.5. Samples were taken at an OD_{600 nm} = 1.5. Data represent the mean of three independent experiments ± SD.