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. 2017 Dec 27;46(5):2600–2612. doi: 10.1093/nar/gkx1287

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© The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 6. — CyaR-dependent regulation of hdeD mRNA is hindered in presence of glucose or sodium pyruvate. β-galactosidase assays using HdeD+65-LacZ (translational) fusions in WT (black), ΔcyaR (white), ΔrprA (gray) and ΔcyaR ΔrprA (dark gray) backgrounds. When cells reached an OD_{600 nm} = 0.5, (A) 0.4% glucose or (B) 0.8% sodium pyruvate was added. (C) β-Galactosidase assays using HdeD+65-LacZ (translational) fusions in ΔcpxR (black) and ΔcpxR ΔcyaR (white) in LB medium supplemented with 0.4% glucose or 0.8% sodium pyruvate. LB medium is used as a control. Samples were taken at an OD_{600 nm} of 2.0. Data represent the mean of three independent experiments ± SD.