Table 2. Identification of potential RprA-regulated mRNAs using MAPS technology.
| Gene | Ratio MS2-RprA/Ctrl | Function/Activity | Reference |
|---|---|---|---|
| nlpD | 2295 | Divisome associated factor | Majdalani, 2001 |
| grcA | 1803 | Pyruvate formate-lyase subunit | This study |
| hdeD | 1655 | Acid-resistance membrane protein | This study |
| rpoS | 1528 | σS factor (σ38) | Majdalani, 2001 |
| focA | 1101 | Formate transporter | This study |
| lrhA | 744 | Transcriptional regulator | This study |
| pal | 569 | Subunit of TolB-Pal complex | This study |
| tolB | 324 | Subunit of TolB-Pal complex | This study |
| cpoB | 103 | Cell division coordinator | This study |
| csgD | 23 | Transcriptional regulator | Mika, 2012 |
| ydaM | 21 | Diguanylate cyclase | Mika, 2012 |
List of most significantly co-purified mRNAs using MS2-RprA as bait in an rne131 ΔrprA background (ratio MS2-RprA/RprA control). New putative target mRNAs are highlighted in gray. Only reads on gene sequences are reported here. The complete list of candidates is available in Supplementary Table S3.