Figure 2.

Rtt109 interacts with Asf1 via its C-terminus. (A) Overlay of ¹⁵N-HSQC spectra of Asf1 (100 μM) in the presence of Rtt109-derived peptides (all at 3 mM) of various lengths. The spectra were acquired in PBS pH 6.2 with 5mM BME at 850 MHz and 298 K. (B) Mapping of the CSPs observed upon addition of 3 mM Rtt109^419–436 onto the Asf1 structure in complex with the H3:H4 dimer (from PDB ID 2HUE). (C) CSP analysis of the spectra in (A). (D) Results of ITC and MST titrations of Asf1 with different peptides and proteins. Experiments were performed in triplicate.