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. 2018 Jan 19;46(5):2335–2346. doi: 10.1093/nar/gky001

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© The Author(s) 2018. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 6. — Overexpression of Esco1 increases the acetylation level of α-tubulin and perturb the spindle assembly. (A) Protein levels of Esco1-Flag in control and Esco1-OE (overexpression) oocytes. The blots were probed with anti-Flag antibody, anti-acetyl-α-tubulin antibody and anti-Gapdh antibody, respectively. (B) Representative images of acetylated α-tubulin in control and Esco1-OE oocytes. Oocytes were immunostained with anti-acetyl-α-tubulin antibody and counterstained with Hoechst to visualize chromosomes. Scale bar, 20 μm. (C) The immunofluorescence intensity was recorded in control and Esco1-OE oocytes. (D) Representative images of spindle morphologies and chromosome alignment in control and Esco1-OE oocytes. Oocytes were immunostained with anti-α-tubulin-FITC antibody to visualize spindles and counterstained with Hoechst to visualize chromosomes. Scale bar, 20 μm. (E) The rate of aberrant spindles was recorded in control and Esco1-OE oocytes. (F) The rate of misaligned chromosomes was recorded in control and Esco1-OE oocytes. (G) Quantitative analysis of PBE rate in control and Esco1-OE oocytes. Data of (C), (E), (F) and (G) were presented as mean percentage (mean ± SEM) of at least three independent experiments. **P < 0.01, ***P < 0.001.