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. 2018 Jan 22;46(5):2370–2379. doi: 10.1093/nar/gky021

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© The Author(s) 2018. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 2. — HU promotes looping. (A) Increasing HU concentration progressively decreased the mean squared extension values of Os-400-O1 or O1-400-O2 tethers from 100 to 50% (right hand Y-axis) simultaneously driving the looping probability from 25 to 80% (left hand Y-axis). (B) Plotting the looping probability as a function of the HU-modified extension of the DNA reveals an inverse relationship for DNA without tension. Application of slight tension reversed the HU-induced contraction and lowered the looping probability. The lower looping probability of tethers under tension in a magnetic tweezer may reflect a lower probability of the tether to adopt conformations with writhe when the bead was rotationally constrained.