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. 2018 Jan 4;46(5):2308–2320. doi: 10.1093/nar/gkx1302

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© The Author(s) 2018. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — The tfeB gene encoding TFEβ is non-essential in H. volcanii. (A) Southern blot of EcoRV digest confirming deletion of tfeB in H2644 and H2645. (B) Deletion of tfeB confers a slow-growth phenotype in H2644 and H2645, versus the parent strain H53. Data from the mean of four repeats (and standard error) is plotted on a log₂ scale, the generation time in exponential phase is shown in bold (exponential growth phase is indicated by straight-line fit between perpendicular marks, R² = 0.99 for all strains). (C) The growth defect conferred by deletion of tfeB in H2644 is complemented by in trans expression of tfeB (eV, empty vector).