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. 2018 Jan 18;46(5):2356–2369. doi: 10.1093/nar/gky017

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© The Author(s) 2018. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 5. — Tests of JMJ705 and WOX11 binding and H3K27me3 levels of selected putative common target genes. (A) ChIP assays with anti-FLAG and anti-IgG of 5 days seedling chromatin isolated from wild type (ZH11) and Flag-tagged WOX11 transgenic plants (OXF1). Pw indicates primers contain WOX11 binding site. Actin was used as an internal control. (B) ChIP assays with anti-H3K27me3 of 5 d seedling chromatin isolated from wild type (HY) and wox11 mutant Actin was used as an internal control. (C) ChIP assays with anti-FLAG and anti-IgG of 5 d seedling chromatin isolated from wild type (ZH11) and JMJ705-FLAG plants (oxJ5–5). The first six genes contain both the WOX11 (Pw) and JMJ705-binding (Pz) sites, and the other three genes (bZIP47,Os11g13890, U2AF) contain only WOX11 binding sites. For ERF3 (with Pw and Pz sites), there was no proper primer for Pw and only Pz was detected and presented in the inner box. Actin was used as an internal control. (D) ChIP assays with anti-H3K27me3 of 5 days seedling chromatin isolated from wild type (ZH11) and oxJ5–5 seedlings. Y-axis means assay-site fold enrichment of the signal from immunoprecipitation over the background. Bar indicates mean ± SD from three replicates. Significances of differences are indicated. *P <0.05; **P <0.01, n, not significant, t-test, two-sided.