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. 2018 Jan 18;46(5):2356–2369. doi: 10.1093/nar/gky017

Figure 6.

Figure 6.

WOX11 modulates JMJ705 binding to targeted genes. ChIP assays with anti-FLAG (anti-IgG as controls) of 5-day-old seedling chromatin isolated from the segregated wild type (WT), the JMJ705-FLAG in wild type background (oxJ5), and JMJ705-FLAG in wox11 background (wox11/oxJ5), from F2 of the crosses between wox11 and oxJ5–5. Seven representative genes were tested. OSH3, OSH15, OSH71, LHY and ERF3 contain both the WOX11 (Pw) and JMJ705-binding (Pz) sites, bZIP47 and Os11g13890 contain only the WOX11-binding site. The Os12g02210 locus that contains only JMJ705-binding site was used as an internal control. Gene structures, Pw and Pz sites were indicated. Three biological replicates of independent chromatin preparations were performed. One of the biological replicate is shown here. The other two biological replicates are in shown in Supplementary Figure S8. Y-axis means assay-site fold enrichment of the signal from immunoprecipitation over the background. Bar = means ± SD from three technical repetitions.