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. 2018 Jan 18;46(5):2356–2369. doi: 10.1093/nar/gky017

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© The Author(s) 2018. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 7. — WOX11-dependent recruitment of JMJ705 to genomic loci. (A) Venn diagram displaying significant overlaps between genes occupied by JMJ705 in oxJ5 and wox11/oxJ5. (B) Mean density of JMJ705 occupancy at all JMJ705-associated genes in oxJ5 (1115 genes) as compared to genes with wox11/oxJ5 (946). Numbers of sequenced tags (Y-axis) per 5% of the genic region (black box) or per 100 bp intervals in the 2-kb genomic region flanking the gene (line, X-axis) are shown. Arrow indicates the direction of transcription. (C) Mean density of JMJ705 occupancy at all JMJ705-associated sites (peaks found in oxJ5) in oxJ5 and wox11/oxJ5. The average signal within 2-kb genomic regions flanking the center of the JMJ705 peaks is shown. (D) The Integrative Genomics Viewer (IGV) views of JMJ705 signal on representative genes in oxJ5 and wox11/oxJ5. Gene structures and name are shown underneath. Red lines represent tested regions in ChIP-qPCR. The scale was identical for different tracks, and the Y-axis scales represent normalized signal of shifted merged MACS tag counts for every 10-bp window. Bar = 1 kb. (E) ChIP-qPCR detection of JMJ705 occupancy with anti-IgG (negative control) and anti-FLAG at genes loci listed in (D) in WT, oxJ5 and wox11/oxJ5 apex. Os05g32290 was used as a negative control. Y-axis means assay-site fold enrichment of the signal from immunoprecipitation over the background.