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. 2018 Mar 19;12(Suppl 2):27. doi: 10.1186/s12918-018-0548-z

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© The Author(s) 2018

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver(http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Analysis workflow. The analysis workflow consists of four steps: 1) the identification of putative coding genes, 2) miRNA detection, 3) predicting mRNA targets of miRNAs and 4) validating genes and miRNAs by RT-PCR. The gray boxes represent external databases used for the annotation of genes and miRNAs