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. 2017 Aug 2;6:ISCB Comm J-1302. [Version 1] doi: 10.12688/f1000research.11352.1

Figure 3. Identification of new PML-RARA fusion transcripts.

Figure 3.

( A) Scheme of PML and RARA genes are shown on the top, with the potential breakpoints. Below is the schematic of the chromosomal translocation, with visualization of the short bcr3 fusion transcript. Gene structures for both PML and RARA genes (ENSEMBL genome browser), and exons involved in new PML-RARA chimera are shown. We assigned a number to exons ( E) of each gene. Bcr3 fusions found in this study are shown below, including the newly found fusion between exon 3 of PML and exon 12 of RARA. ( B) New RARA-PML antisense fusion transcript. Schematic of RARA (blue) and PML (red) antisense transcript positions relative to their sense transcript, and the resulting antisense fusion transcript, are shown below. ( C) Identification of selected PML-RARA transcript junctions in acute promyelocytic leukemia samples and NB4 cells by qPCR. ( D) Hypothetical incidence of PML-RARA fusion transcript on protein domains. Sanger sequencing results of the 3-12 PML-RARA fusion is shown on the top. The hypothetical protein product is presented below, as well as the protein from the exon 3–9 fusion.