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. 2018 Mar 14;9:327. doi: 10.3389/fpls.2018.00327

FIGURE 3.

FIGURE 3

Effects of mechanical wounding on chlorophyll fluorescence parameters and chlorophyll content in the local and the systemic leaves of wheat under freezing stress. The maximal photochemical efficiency of photosystem II (PSII) as indicated by Fv/Fm (A,B), total chlorophyll content expressed in mg g-1 FW (fresh weight) (C), photochemical activity of PSII as indicated by Fv′/Fm′(D), the non-photochemical quenching as indicated by NPQ (E) and the quantum yield of PSII as indicated by ΦPSII (F). The local (fifth) leaves were separately pre-treated with distilled water, 5 mM DMTU or 100 μM DPI prior to wounding. At 10 days after wounding, the seedlings were exposed to freezing stress for 24 h. Chlorophyll fluorescence parameters were observed on the systemic (sixth) leaf as area of interest after 24 h recovery. Data are mean values ± SD of three independent replicates. Means denoted by different letters indicated significant difference at P < 0.05 according to Tukey’s test. Bar, 10 mm.