Figure 2.

Epicardial fiber bundle consisting of nonvaricose angiotensin (Ang) II-positive (red, a) and tyrosine hydroxylase (TH)-positive fibers (green, b). (c) An overlay picture with co-localization of Ang II and TH immunoreactivity in numerous fibers (yellow). The arrow points to an exclusively Ang II-positive fiber next to adjacent exclusively TH-positive fibers. The arrowhead indicates a fiber with co-localization of Ang II and TH. There are Schwann cell nuclei visible between the fibers. Dual channel laser scanning microscopy. Staining of nuclear DNA was with 4′,6′-diamidino-2-phenylindole (blue). Scale bar represents 10 µm.