Figure 4.

(a) Water intake, urinary volume, and food intake, and (b) activity in normotensive (NT) or 2K1C rats that received intra-NTS injections of AAV2-CBA-eGFP or AAV2-CBA-MIF vectors 3 weeks after renal clipping (arrows). Activity mean was generated from a period of 2 hours during the light and the dark periods, once a week, for 7 weeks. The results are presented as means ± SEM. In panel a the number of animals/group are: NT-GFP, n = 4; NT-MIF, n = 3; 2K1C-GFP, n = 4; 2K1C-MIF, n = 4. In panel b, the number of animals are the same as in Figure 2; *P < 0.05 vs. NT groups; ^#P < 0.05 vs. 2K1C-GFP group; ^&P < 0.05 vs. baseline (0); 2-way ANOVA, followed by Student–Newman–Keuls test. Abbreviations: ANOVA, analysis of variance; MIF, macrophage migration inhibitory factor; NTS, nucleus of the solitary tract.