Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Mar 20;15:90. doi: 10.1186/s12974-018-1128-2

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s). 2018

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

PMC Copyright notice

Fig. 11 — Blocking LAR and PTPσ receptors has no effect on formation of astrocytic scar and CSPGs following SCI. a To study scar formation under ILP and ISP treatment, we examined GFAP protein expression by Western blotting at 1, 3, 5, 7, and 14 days post-injury. As anticipated, GFAP protein expression was significantly increased following injury compared to uninjured animals. However, ILP and ISP treatment had no significant effect on the expression of GFAP at any time-points after SCI. b Using immunohistochemistry, we also studied astrocytic scar in SCI lesion. Our quantitative immunodensity analysis in the injured spinal cord tissue at the chronic 28 days post-SCI also showed no significant differences in the levels of GFAP under ILP/ISP treatment compared to vehicle SCI control group. c Slot blot analysis of CS56 expression at 1, 3, 5, 7, and 14 days post-SCI demonstrated a significant increase CSPGs following injury. Similar to GFAP, ILP and ISP treatment had no effect on the deposition of CSPGs following injury. d CS56 expression was additionally measured using immunohistochemistry at different distances to injury epicenter at 28 days post-SCI. ILP/ISP treatment showed no significant differences in the levels of CS56 compared to vehicle SCI control group. The data show the mean ± SEM, *p < 0.05, **p < 0.001, one-way ANOVA (a, c), two-way ANOVA (b–d), N = 4–6/group