Fig. 2.

Evidence for NLRP3 expression within mouse myofibres. Wild-type mice (WT) and Nlrp3-KO mice were challenged by intraperitoneal (ip) LPS or received vehicle only (no LPS), and tibialis anterior muscles were sampled 24 h later. a Immunochemistry was performed with a specific antibody directed against NLRP3. Clusters of staining, shown by arrows in the magnification inset, were detected in myofibres of WT mice after LPS, while there was no labelling in Nlrp3-KO mice. Mice receiving vehicle only are shown for comparison. Scale bar = 50 μm for all images including the inset. Representative sections for six mice per group are presented. b Western blot analysis was also performed in the different groups of mice; data were normalized to actin levels and presented as percentages of WT without LPS. Values are means ± standard error of the mean (SEM) for three to six mice per group. **P < 0.01 for the effect of LPS