Fig. 7.

Effects of NLRP3 depletion on components of the inflammasome complex in mdx muscles. Tibialis anterior muscles were sampled from WT mice, Nlrp3-KO mice, mdx mice and mdx/Nlrp3-KO mice. Members of the inflammasome complex (NLRP3, ASC and caspase-1) were measured at the mRNA and/or protein levels. mRNA levels were normalized to cyclophilin and presented as relative expression compared to WT values. Protein quantification was performed by Western blot. NLRP3 and total caspase-1 levels (45 kDa proenzyme and its active cleaved subunits) were normalized to actin levels and presented as percentages of WT values. Caspase activity (activated 20 kDa subunit) was expressed as percentage of total forms. Results are means ± SEM for eight to ten (mRNA) or six (protein) mice per group. #P < 0.05, ###P < 0.001 vs WT; **P < 0.01 for indicated conditions