Fig. 8.

Immunodetection of inflammation and oxidative stress markers in mdx muscles with Nlrp3 abrogation. Tibialis anterior muscles were sampled from WT mice, Nlrp3-KO mice, mdx mice and mdx mice that were also Nlrp3-deficient (mdx/Nlrp3-KO). Immunodetection was performed with specific antibodies directed against NLRP3, three pro-inflammatory cytokines (IL-1β, IL-18 and TNFα) and one oxidative stress marker (peroxiredoxin 3, PRDX3). IL-1β antibody was specific for the mature form of the protein, while IL-18 antibody recognized its total form. Representative sections for six mice per group are shown. Scale bar = 50 μm