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. Author manuscript; available in PMC: 2018 Aug 30.
Published in final edited form as: J Am Chem Soc. 2017 Aug 16;139(34):11670–11673. doi: 10.1021/jacs.7b05125

Figure 4.

Figure 4

A functional Herceptin-Fab-fluorophore conjugate created using 5HTP-directed CRACR. (A) ESI-MS analysis of the wild-type Fab (expected mass 47752 Da), Fab-169-5HTP (expected mass 47826 Da), and the conjugate between Fab-169-5HTP and 7 (expected mass 48186 Da). (B) SDS-PAGE followed by Coomassie staining (top) and fluorescence imaging (bottom) reveals selective fluorescence-labeling of Fab-169-5HTP, but not wild-type Fab by 7. (C) The conjugate between 7 and Fab-169-5HTP conjugate can bind the Her2-overexpressing SK-BR-3 cells, as shown by FACS analysis. Percentage of cells fluorescing within the R4 gate is shown in each case.