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The principal of single cross-over gene disruption. (A) Maps of pUX1, pUX2 and pUX3. (B) The GPL-transporter locus, consisting of mmpS4, mmpL4a and mmpL4b in M. abscessus (Top). Disruption of mmpL4a through integration of pUX1-mmpL4a (Middle). Replacement of the mmpS4-mmpL4a-mmpL4b operon promoter with an acetamide-inducible promoter through integration of pUX3 (bottom).
