Table 2.

Frequency of plasmid uptake by homologous recombination.

Size of cloned fragment in pUX1/3 (bp)	100b	300c	500b	1,000b	1,500b
N° Red fluorescent colonies	0	3	5	43	147
% Colonies PCR positive	0	100	100	100	100
% Efficiencya	0	100	100	100	100
Transformation efficiency	1.45 × 104 CFU/μg DNA

^aPercentage of total number of colonies that are red fluorescent and PCR positive.

^bCloned into pUX1.

^cCloned into pUX3.