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Replication of reassortant viruses in MDCK cells with or without TPCK-trypsin. V1E1: RG-17SF003V1E1 with initial growth in Vero cells followed by 1 passage in eggs; V1E5: RG-17SF003V1E5 with initial growth in Vero cells followed by 5 passages in eggs; PR8: A/Puerto Rico/8/34 generated by RG. The original viruses were diluted from 10−1to 10−5 and each diluted viruses were inoculated in MDCK cells. Mock cells were inoculated with PBS.
