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. 2018 Feb 1;14(3):532–539. doi: 10.1080/21645515.2017.1419109

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© 2018 The Author(s). Published with license by Taylor & Francis

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.

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Figure 4. — A Total protein content (left Y axis) and HA proportion (right Y axis). Total protein content per 10 eggs of each virus. HA proportion was determined using the following equation: HA1+HA2 /HA1+HA2+NP+M. B SDS-PAGE profile of various influenza viruses using the same sample amount. M: Marker (KD). PR8: A/Puerto Rico/8/34 generated by RG; V1E1: RG-17SF003V1E1 with initial rescued in Vero cells followed by 1 passage growth in eggs; V1E5: RG-17SF003V1E5 with initial rescued in Vero cells followed by 5 passages growth in eggs. The data was the results of two independent tests and analyzed by two-way ANOVA using GraphPad Prism 5 software package (version 5.0).** represents p < 0.01; *** represents p < 0.001.