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Six-to-eight-week-old BALB/c mice (n = 6) and SD rats (n = 6) were twice immunized ID or IM with 3, 9, or 15 µg of HA of the split H7N9 influenza vaccine at 2-week intervals, and serum samples were collected at 2, 3, and 4 weeks post-boosting for HI antibody measurements (A, B). Mice and rats were immunized with the H7N9 influenza vaccine at 0 and 14 days, and serum HI antibody titers and IgG subtypes were assayed at 2 and 4 weeks post-boosting (C).
