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. 2017 Nov 17;14(3):508–517. doi: 10.1080/21645515.2017.1377871

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Figure 3. — Representative CID and HCD spectra of H1 glycopeptides. (A) Annotated HCD spectrum of glycopeptide ESSWPNHTVTGVSASCSHNGK. The spectrum exhibits the diagnostic oxonium ion at 204.08 m/z and the presence of b and y ions derived from backbone fragmentation. (B) Annotated CID spectrum of glycopeptide ESSWPNHTVTGVSASCSHNGK with an attached glycan with a HexNAc(4)Hex(5)Fuc(1) composition. Sequential glycan fragmentation is predominant in the spectrum and allows for the validation of predicted glycan composition. The presence of a core fucose can be confirmed by the detection of ion Pep+2HexNAc-Fuc in both the HCD and CID spectra.