Table 2.
Microheterogeneity of A/New Caledonia/20/1999 H1.
| Position of glycan attachment within the protein sequence |
Predicted glycan composition |
Ion detected (m/z) |
Glycan mass (Da) |
Δm/z (Da) |
| Site 1: Asparagine 28 (NSTD) Stalk | HexNAc(5)Hex(3) | 1,389.59 (+6) | 1,501.55 | −0.3369 |
| Site 2: Asparagine 40 (NVTV) Stalk | HexNAc(5)Hex(8)Fuc(1) | 1,474.63 (+3) | 2,457.87 | 0.0079 |
| HexNAc(5)Hex(6) | 988.68 (+4) | 1,987.71 | 0.00022 | |
| Site 3: Asparagine 71 (NCSV) Side of the head | HexNAc(3)Hex(5)NeuGc(1) | 1,564.69 (+3) | 1,726.59 | −0.36158 |
| Site 4: Asparagine 104 (NGTC) Side of the head | HexNAc(7)Hex(3)Fuc(1) | 1,389.85 (+4) | 2,053.77 | 0.27418 |
| HexNAc(2)Hex(3) | 1,081.43 (+4) | 892.31 | −0.001997 | |
| Site 5: Asparagine 142 (NHTV) Top of the head | HexNAc(6)Hex(6)Fuc(1) | 1,149.22 (+4) | 2,336.85 | 0.00096 |
| HexNAc(6)Hex(5) | 1,054.67 (+4) | 2,028.74 | 0.24239 | |
| HexNAc(6)Hex(4)NeuAc(1) | 1,104.64 (+4) | 2,157.78 | 0.25827 | |
| HexNAc(6)Hex(4)Fuc(1) | 1,068.44 (+4) | 2,012.74 | 0.24967 | |
| HexNAc(6)Hex(4) | 953.60 (+5) | 1,866.68 | 0.00167 | |
| HexNAc(6)Hex(3)Fuc(1)NeuAc(1) | 1,466.57 (+3) | 2,141.78 | −0.35536 | |
| HexNAc(6)Hex(3)Fuc(2) | 1,046.42 (+4) | 1,996.75 | −0.00844 | |
| HexNAc(6)Hex(3) | 991.16 (+4) | 1,704.63 | 0.00043 | |
| HexNAc(5)Hex(6)Fuc(2) | 1,134.72 (+4) | 2,279.82 | 0.00087 | |
| HexNAc(5)Hex(6)Fuc(1) | 1,098.45 (+4) | 2,133.77 | 0.00237 | |
| HexNAc(5)Hex(6) | 1,061.70 (+4) | 1,987.71 | −0.24352 | |
| HexNAc(5)Hex(5)Fuc(3) | 1,130.96 (+4) | 2,263.83 | 0.00054 | |
| HexNAc(5)Hex(5)Fuc(2) | 1,094.45 (+4) | 2,117.77 | 0.00123 | |
| HexNAc(5)Hex(5)Fuc(1) | 1,057.94 (+4) | 1,971.71 | 0.0035 | |
| HexNAc(5)Hex(5) | 1,021.42 (+4) | 1,825.66 | −0.00022 | |
| HexNAc(5)Hex(4)NeuAc(1) | 1,035.43 (+4) | 1,954.70 | −0.24191 | |
| HexNAc(5)Hex(4)Fuc(1) | 1,356.23 (+3) | 1,809.66 | 0.00052 | |
| HexNAc(5)Hex(4) | 980.91 (+4) | 1,663.60 | −0.00032 | |
| HexNAc(5)Hex(4)Fuc(2) | 1,955.72 (+4) | 1,930.69 | 0.00039 | |
| HexNAc(5)Hex(3)Fuc(1)NeuGc(1) | 1,404.24 (+3) | 1,954.70 | −0.32956 | |
| HexNAc(5)Hex(3)Fuc(1) | 976.91 (+4) | 1,647.61 | −0.00067 | |
| HexNAc(4)Hex(7) | 1,402.13 (+3) | 1,948.68 | 0.32814 | |
| HexNAc(4)Hex(6)NeuGc(1) | 1,450.25 (+4) | 2,091.72 | 0.33191 | |
| HexNAc(4)Hex(6)NeuAc(1) | 1,444.92 (+3) | 2,075.72 | 0.33262 | |
| HexNAc(4)Hex(6)Fuc(2) | 1,084.19 (+4) | 2,076.75 | 0.00188 | |
| HexNAc(4)Hex(6)Fuc(1) | 1,047.93 (+4) | 1,930.69 | 0.2478 | |
| HexNAc(4)Hex(6) | 1,324.20 (+3) | 1,784.63 | 0.00147 | |
| HexNAc(4)Hex(5)NeuGc(1) | 1,047.18 (+4) | 1,926.67 | 0.00257 | |
| HexNAc(4)Hex(5)NeuAc(1) | 1,367.21 (+3) | 1,931.67 | 0.3165 | |
| HexNAc(4)Hex(5)Fuc(3) | 1,079.95 (+4) | 2,060.75 | 0.00052 | |
| HexNAc(4)Hex(5)Fuc(2) | 1,043.68 (+4) | 1,914.69 | 0.00044 | |
| HexNAc(4)Hex(5)Fuc(1) | 1,007.41 (+4) | 1,768.63 | 0.24923 | |
| HexNAc(4)Hex(5) | 970.65 (+4) | 1,622.58 | −0.00094 | |
| HexNAc(4)Hex(4)Fuc(2)NeuGc(1) | 1,061.69 (+4) | 2,059.73 | −0.24548 | |
| HexNAc(4)Hex(4)Fuc(2)NeuAc(1) | 1,057.69 (+4) | 2,043.74 | −0.24382 | |
| HexNAc(4)Hex(4)Fuc(1)NeuAc(1) | 1,021.17 (+4) | 1,897.68 | −0.24484 | |
| HexNAc(4)Hex(4)NeuAc(1) | 1,003.16 (+4) | 1,751.62 | 0.2533 | |
| HexNAc(4)Hex(4)Fuc(2) | 1,003.17 (+4) | 1,752.64 | 0.00223 | |
| HexNAc(4)Hex(4)Fuc(1) | 901.58 (+5) | 1,606.58 | 0.00127 | |
| HexNAc(4)Hex(4) | 929.89 (+4) | 1,460.52 | 0.00082 | |
| HexNAc(4)Hex(3)Fuc(3) | 981.16 (+4) | 1,736.64 | −0.00291 | |
| HexNAc(4)Hex(3)Fuc(1) | 926.14 (+4) | 1,444.53 | 0.00019 | |
| HexNAc(3)Hex(6)Fuc(1)NeuGc(1) | 1,431.24 (+3) | 2,034.70 | 0.32942 | |
| HexNAc(3)Hex(6)Fuc(1)NeuAc(1) | 1,402.56 (+3) | 2,018.70 | 0.33363 | |
| HexNAc(3)Hex(6)NeuGc(1) | 1,037.41 (+4) | 1,888.64 | 0.24807 | |
| HexNAc(3)Hex(6)Fuc(1) | 996.91 (+4) | 1,727.61 | 0.00239 | |
| HexNAc(3)Hex(6) | 1,280.18 (+3) | 1,581.55 | −0.01264 | |
| HexNAc(3)Hex(5)Fuc(1) | 956.39 (+4) | 1,565.56 | 0.00357 | |
| HexNAc(3)Hex(5) | 919.8 (+4) | 1,419.50 | −0.00045 | |
| HexNAc(3)Hex(4)Fuc(2) | 915.88 (+4) | 1,549.56 | 0.00085 | |
| HexNAc(3)Hex(4)Fuc(1) | 915.64 (+4) | 1,403.50 | 0.00039 | |
| HexNAc(3)Hex(3)Fuc(1) | 875.37 (+4) | 1,241.45 | −0.00017 | |
| HexNAc(3)Hex(3) | 838.85 (+4) | 1,095.39 | 0.00015 | |
| HexNAc(2)Hex(5) | 869.11 (+4) | 1,216.42 | 0.00035 | |
| HexNAc(2)Hex(6) | 909.62 (+4) | 1,378.47 | 0.00027 | |
| HexNAc(2)Hex(7) | 950.13 (+4) | 1,540.52 | −0.00177 | |
| HexNAc(2)Hex(8) | 1,320.51 (+4) | 1,702.58 | −0.01245 | |
| Site 6: Asparagine 177 (NLSK) Top of the head |
HexNAc(5)Hex(4)Fuc(1)NeuAc(1) | 1,553.65 (+2) | 2,100.76 | −0.0034 |
| HexNAc(4)Hex(6) | 1,166.17 (+3) | 1,784.63 | 0.00055 | |
| HexNAc(2)Hex(8) | 1,354.56 (+2) | 1,702.58 | 0.00196 | |
| HexNAc(2)Hex(7) | 1,273.53 (+2) | 1,540.52 | 0.00127 | |
| HexNAc(2)Hex(6) | 1,193.00 (+2) | 1,378.47 | 0.0015 | |
| HexNAc(2)Hex(5) | 1,111.98 (+2) | 1,216.42 | 0.00069 | |
| HexNAc(2) | 585.06 (+4) | 406.15 | −0.00064 | |
| Site 7: Asparagine 304 (NSSL) Stalk | HexNAc(6)Hex(4) | 1,657.73 (+4) | 1,866.68 | 0.25101 |
| HexNAc(6)Hex(3) | 1,621.22 (+4) | 1,704.63 | 0.25377 | |
| HexNAc(4)Hex(5)NeuAc(1) | 1,686.49 (+4) | 1,913.67 | −0.24401 | |
| HexNAc(4)Hex(4)NeuGc(1) | 1,649.97 (+4) | 1,767.61 | −0.25517 | |
| HexNAc(3)Hex(6)NeuAc(1) | 1,659.22 (+4) | 1,872.64 | 0.25161 | |
| HexNAc(3)Hex(4)Fuc(1)NeuAc(1) | 1,614.70 (+4) | 1,694.60 | 0.2467 | |
| Site 8: Asparagine 498 (NGTY) Stalk | HexNAc(8)Hex(8)Fuc(1) | 1,342.18 (+3) | 3,067.11 | −0.00606 |
| HexNAc(7)Hex(7)Fuc(1)NeuAc(2) | 1,414.88 (+3) | 3,284.17 | 0.3354 | |
| HexNAc(6)Hex(6) | 1,050.08 (+3) | 2,190.79 | 0.00079 | |
| HexNAc(6)Hex(5)Fuc(1)NeuAc(2)NeuGc(1) | 1,341.19 (+3) | 3,064.07 | 0.02 | |
| HexNAc(6)Hex(3)Fuc(2)NeuGc(1) | 1,471.89 (+3) | 2,303.84 | −0.34158 | |
| HexNAc(5)Hex(7) | 1,415.21 (+3) | 2,149.76 | −0.33692 | |
| HexNAc(5)Hex(5)Fuc(1)NeuAc(1) | 1,458.23 (+3) | 2,262.81 | −0.33147 | |
| HexNAc(5)Hex(5)NeuGc(1) | 1,409.55 (+3) | 2,132.75 | −0.32933 | |
| HexNAc(5)Hex(5)Fuc(1) | 1,465.08 (+2) | 1,971.71 | 0.00223 | |
| HexNAc(5)Hex(5) | 1,307.84 (+3) | 1,825.66 | 0.00225 | |
| HexNAc(5)Hex(4)Fuc(1)NeuAc(1) | 1,401.21 (+3) | 2,100.76 | −0.32961 | |
| HexNAc(5)Hex(4)Fuc(1)NeuGc(1) | 1,409.55 (+3) | 2,116.75 | −0.32763 | |
| HexNAc(5)Hex(4)NeuGc(1) | 1,384.87 (+3) | 1,970.69 | −0.00099 | |
| HexNAc(5)Hex(3)Fuc(1)NeuGc(1) | 1,355.53 (+3) | 1,945.70 | −0.32857 | |
| HexNAc(4)Hex(6) | 1,293.83 (+3) | 1,784.63 | −0.00351 | |
| HexNAc(4)Hex(5)Fuc(1)NeuGc(1) | 1,390.54 (+4) | 2,075.72 | −0.32938 | |
| HexNAc(4)Hex(5)NeuGc(1) | 1,366.19 (+3) | 1,929.67 | 0.00128 | |
| HexNAc(4)Hex(5)Fuc(3) | 1,386.20 (+3) | 2,060.75 | −0.00084 | |
| HexNAc(4)Hex(5)Fuc(2) | 1,006.74 (+3) | 1,914.69 | 0.00054 | |
| HexNAc(4)Hex(5)Fuc(1) | 1,288.83 (+3) | 1,768.63 | −0.00049 | |
| HexNAc(4)Hex(5) | 1,101.78 (+3) | 1,622.58 | 0.00172 | |
| HexNAc(4)Hex(4)Fuc(2)NeuAc(1) | 1,385.21 (+3) | 2,043.74 | −0.32697 | |
| HexNAc(4)Hex(4)Fuc(1)NeuGc(1) | 1,360.86 (+3) | 1,913.67 | 0.32938 | |
| HexNAc(4)Hex(4)Fuc(1)NeuAc(1) | 1,336.52 (+3) | 1,987.68 | −0.33167 | |
| HexNAc(4)Hex(4)Fuc(1) | 9,43.87 (+4) | 1,606.58 | −0.00836 | |
| HexNAc(3)Hex(6)Fuc(1)NeuGc(1) | 1,382.18 (+3) | 2,034.70 | −0.34415 | |
| HexNAc(3)Hex(6)Fuc(1) | 1,343.03 (+2) | 1,727.61 | 0.00136 | |
| HexNAc(3)Hex(5)NeuGc(1) | 1,298.49 (+3) | 1,726.59 | −0.00482 | |
| HexNAc(3)Hex(4)Fuc(1)NeuAc(1) | 1,287.83 (+2) | 1,694.60 | 0.00233 | |
| HexNAc(3)Hex(4)NeuAc(1) | 1,239.15 (+3) | 1,548.54 | 0.00166 | |
| HexNAc(3)Hex(4)Fuc(2) | 1,239.15 (+3) | 1,549.56 | −0.01108 | |
| HexNAc(3)Hex(4) | 1,107.95 (+2) | 1,257.44 | 0.00098 | |
| HexNAc(2)Hex(8) | 1,330.49 (+2) | 1,702.58 | −0.02006 |