TABLE 5.
Strains used in this studya
| Strain | Relevant genotype | Result(s) of mutation | Characteristic(s) | Source or reference |
|---|---|---|---|---|
| MG1655 | F− λ− ΔilvG rfb-50 rph-1 | ATCC 700926 | ||
| CAR | soxR (G4275900T); 95-kb deletion (positions 3623620–3718650) | Predicted SoxR missense mutation D137Y; 1 large chromosomal deletion, Δ(yhhJ-mokA) | MG1655 10-day carvacrol selection | 11 |
| CIT | gyrB (−GGT3877671); marR (+A1617171); intergenic eptB-yhjX (A3708613C) | GyrB in-frame ΔG157, MarR E10 frameshift | MG1655 10-day citral selection | 11 |
| LIM | acrR (+C485340); 4-kb deletion (positions 1972710–1976520); 11-kb deletion (positions 2800250–2811150) | ArcR F119 frameshift; 2 large chromosomal deletions, Δ(cheA-insA) and Δ(nrdE-enrB) | MG1655 10-day (+)-limonene oxide selection | 11 |
| CAG12156 | MG1655 uvrC279::Tn10 | 60 | ||
| CAG12164 | MG1655 malF3089::Tn10 | 60 | ||
| CAG18592 | MG1655 zie-3163::Tn10Kan | 60 | ||
| BC010 | soxR rev. WT (MG1655) | CAR, soxR rev. WT, Tetr nearby; P1(CAG12164) × CAR | This study | |
| BC020 | gyrB rev. WT (MG1655) | CIT, gyrB rev. WT, Kanr nearby; P1(CAG18592) × CIT | This study | |
| BC021 | gyrB (−GGT3877671) | GyrB in-frame ΔG157 | CIT, gyrB Kanr nearby; P1(CAG18592) × CIT | This study |
| BC022 | gyrB (−GGT3877671) | GyrB in-frame ΔG157 | MG1655, gyrB (−GGT157) Kanr nearby; P1(BC021) × MG1655 | This study |
| BC030 | 4-kb deletion rev. WT (MG1655) | LIM, 4-kb deletion rev. WT, Tetr nearby; P1(CAG12156) × LIM | This study | |
| BC031 | 4-kb deletion (positions 1972710–1976520) | 1 large chromosomal deletion, Δ(cheA-insA) | LIM, 4-kb deletion (positions 1972710–1976520) Tetr nearby; P1(CAG12156) × LIM | This study |
| BC032 | 4-kb deletion (positions 1972710–1976520) | 1 large chromosomal deletion, Δ(cheA-insA) | MG1655, 4-kb deletion (positions 1972710–1976520) Tetr nearby; P1(BC031) × MG1655 | This study |
a
rev., reverted to.