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Effects of dry-heat treatment on B. subtilis spores. (A) Percentages of spores that survived and retained CaDPA as a function of treatment time at 140°C were determined as described in Materials and Methods. (B) Raman spectra of single spores of B. subtilis that retained CaDPA after various dry-heat treatment times. (C) Raman spectra of single spores of B. subtilis in the protein amide band regions. Band intensities are given in arbitrary units (AU).
