FIG 5.

Dodecylamine germination of B. subtilis spores with and without dry-heat treatment. (A) Germination after various dry-heat treatment times with dodecylamine, with >300 spores examined for each condition as described in Materials and Methods. (B, C, D) Dodecylamine germination of 10 single spores after dry-heat treatment at 140°C for (B) 0, (C) 30, or (D) 60 min, respectively. Germination was followed as described in Materials and Methods. Image intensities are given in arbitrary units (AU).