FIG 7.

Germination, outgrowth, and growth of individual B. subtilis spores with and without dry-heat treatment. Time-lapse images of single B. subtilis spores given various treatments at 140°C that were then incubated in LB medium agar at 37°C were recorded as described in Materials and Methods. (A) Time-lapse images of single spores without dry-heat treatment. (B, C) Time-lapse images of single spores after dry-heat treatments at 140°C for 7 min and 30 min, respectively. The two arrows in A and B indicate two individual spores that germinated, outgrew, and began vegetative growth. Note that in C, spores that germinated did not go through outgrowth, even after 15 h. Bar, 5 μm.