Skip to main content
. 2018 Mar 19;84(7):e02618-17. doi: 10.1128/AEM.02618-17

TABLE 3.

T1, Tlag, ΔTleakage, ΔTrelease, and ΔTlys for glucose germination of B. megaterium spores with or without dry-heat treatmenta

Treatment condition(s) No. of spores examined (% spore germination) T1 (mean ± SD) (min) Tlag (mean ± SD) (min) ΔTleakage (mean ± SD) (min) ΔTrelease (mean ± SD) (min) ΔTlys (mean ± SD) (min)
Control (untreated) 582 (79.2) 26.5 ± 25.4 28.9 ± 28.2 2.4 ± 1.8 3.3 ± 0.8 7.7 ± 4.5
140°C, 30 min 797 (70.0) 19.9 ± 17.5 22.5 ± 16.5 2.6 ± 1.5 3.1 ± 1.1 11.6 ± 6.1
140°C, 60 min 526 (4.6) 25.3 ± 20.3 27.5 ± 32.8 2.2 ± 1.9 5.2 ± 1.8 29.9 ± 16.2
a

B. megaterium spores with or without dry-heat treatment were germinated with glucose for 2 h, and spore germination parameters were determined as described in Materials and Methods. Data from approximately 100 individual spores that contained CaDPA and germinated were used to calculate the kinetic parameters of germination.