TABLE 3.
Treatment condition(s) | No. of spores examined (% spore germination) | T1 (mean ± SD) (min) | Tlag (mean ± SD) (min) | ΔTleakage (mean ± SD) (min) | ΔTrelease (mean ± SD) (min) | ΔTlys (mean ± SD) (min) |
---|---|---|---|---|---|---|
Control (untreated) | 582 (79.2) | 26.5 ± 25.4 | 28.9 ± 28.2 | 2.4 ± 1.8 | 3.3 ± 0.8 | 7.7 ± 4.5 |
140°C, 30 min | 797 (70.0) | 19.9 ± 17.5 | 22.5 ± 16.5 | 2.6 ± 1.5 | 3.1 ± 1.1 | 11.6 ± 6.1 |
140°C, 60 min | 526 (4.6) | 25.3 ± 20.3 | 27.5 ± 32.8 | 2.2 ± 1.9 | 5.2 ± 1.8 | 29.9 ± 16.2 |
B. megaterium spores with or without dry-heat treatment were germinated with glucose for 2 h, and spore germination parameters were determined as described in Materials and Methods. Data from approximately 100 individual spores that contained CaDPA and germinated were used to calculate the kinetic parameters of germination.