FIG 1.
Expression of PndvB-gfp reporters in planktonic cultures. Cultures of the stable ndvB-gfp reporter (PA14/pSC01), the ASV-tagged reporter (PA14/pAH08), and a negative control (PA14) were diluted (OD600 = 0.05) in M63-arginine medium and incubated at 37°C with shaking. Samples were removed after 6 h (exponential phase) and 12 h (stationary phase) for phase-contrast (left panels) and fluorescence (right panels) microscopy at a magnification of ×630. The brightness was increased in each of the fluorescent panels for image clarity. The images are representative of at least three replicate cultures.