FIG 3.
Expression of PndvB-gfp reporters in biofilms. Biofilms were grown at the air-liquid interface of coverslips partially submerged in M63-arginine medium (coverslip biofilms) and on semipermeable membranes on M63-arginine agar plates (colony biofilms). The coverslip biofilms were rinsed and the coverslips placed on a slide for phase-contrast (left panels) and fluorescence (right panels) microscopy at a magnification of ×630. The colony biofilms were stamped onto a slide for imaging. The images are representative of those from at least three replicate biofilm cultures.