FIG 5.

RpoS is required for the upregulation of ndvB expression observed in stationary-phase and biofilm cells. The expression of ndvB (relative to that in exponential-phase wild-type cultures) was assessed by qPCR in wild-type (WT) PA14 and ΔrpoS strains grown planktonically to exponential (E) or stationary (S) phase or as biofilms (B). Data are expressed as means + SEMs for three biological replicates assayed in three technical replicates. Statistical significance was determined using one-way analysis of variance (ANOVA) with Dunnett's test (the exponential-phase wild type was used as the comparison control).