FIG 7.
The ndvB promoter contains a sequence that matches the RpoS consensus binding site. (A) Comparison of the proposed −10 RpoS binding site in the ndvB promoter to the consensus −10 RpoS elements independently determined by Schuster et al. (38) and by Schulz et al. (39). Sequence identity is shown using asterisks. (B) Comparison of the proposed −10 RpoS binding site in the ndvB promoter with the mutated site in the PndvB-mut-lacZ reporter. (C) β-Galactosidase activity of promoterless lacZ negative control (lacZ), PndvB-lacZ, and PndvB-mut-lacZ reporter constructs that were chromosomally integrated into wild-type PA14 at the attTn7 site. Data are expressed as mean + SEMs for three biological replicates. Statistical significance was determined using one-way ANOVA with Dunnett's test (promoterless lacZ was used as the comparison control).