TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Description | Reference or source |
|---|---|---|
| L. plantarum strains | ||
| IFO3070 | ||
| IFO3070(pLES-PA-A) | IFO3070 transformed with pLES-PA-A | This study |
| IFO3070(pLES-PA-B) | IFO3070 transformed with pLES-PA-B | This study |
| IFO3070(pLES-PA-C) | IFO3070 transformed with pLES-PA-C | This study |
| IFO3070(pLES-PA-D) | IFO3070 transformed with pLES-PA-D | This study |
| IFO3070(pLES-PA-E) | IFO3070 transformed with pLES-PA-E | This study |
| IFO3070(pLES-PA-F) | IFO3070 transformed with pLES-PA-F | This study |
| IFO3070(pLES-breA–H) | IFO3070 transformed with pLES-breA–H | This study |
| IFO3070(pLES-breA–HΔbreD) | IFO3070 transformed with pLES-breA–HΔbreD | This study |
| IFO3070(pLES-breA–HΔbreG) | IFO3070 transformed with pLES-breA–HΔbreG | This study |
| Plasmids | ||
| pET-28a(+) | E. coli expression vector; Kmr | Novagen |
| pET-28a(+)/breD | pET-28a(+) harboring 0.2-kb NdeI-XhoI fragment containing entire breD with N-terminal His6-tag | This study |
| pET-28a(+)/N-breG | pET-28a(+) harboring 0.3-kb NdeI-XhoI fragment containing N-terminal region of breD with N-terminal His6-tag | This study |
| pLES003 | E. coli-LAB shuttle vector; Ampr Ermr | 8 |
| pLES003-b | The replication origin region for LAB (AatII fragment) of this plasmid is reversed from that of pLES003 | This study |
| pLES-PA | pLES003-b lacking lacZα, CAP-binding site, and lac operator | This study |
| pLES-PA-A | pLES-PA containing β-gal gene (PLb) | This study |
| pLES-PA-B | pLES-PA containing β-gal gene (PbreED) | This study |
| pLES-PA-C | pLES-PA containing β-gal gene (PbreED) and breD (PLb) | This study |
| pLES-PA-D | pLES-PA containing β-gal gene (PbreBC) | This study |
| pLES-PA-E | pLES-PA containing β-gal gene (PbreBC) and breG (PbreG) | This study |
| pLES-PA-F | pLES-PA containing β-gal gene and breD (PbreBC, polycistronic) | This study |
| pLES-breA–H | pLES003-b harboring 10-kb fragment containing entire brevicin 174A synthetic gene cluster | This study |
| pLES-breA–HΔbreD | pLES-breA–H containing 3-bp substitution mutation (positions +13 to +15 relative to breD start codon) | This study |
| pLES-breA–HΔbreG | pLES-breA–H containing 3-bp substitution mutation (positions +16 to +18 relative to breG start codon) | This study |