FIG 5.

Effect of the ptsG, miaA, or ridA deletion on growth, ATP level, and GlyA activity. (A) The ΔyggS, Δggt, ΔyggS Δggt ΔptsG, ΔyggS Δggt ΔmiaA, or ΔyggS Δggt ΔridA strain harboring pGshA*B was grown for 24 h by cultivation method B with 1 mM each Glu, 2-AB, and Gly. The OD₆₀₀ of the cultures was monitored every 30 min using an OD-Monitor C&T apparatus (Taitec Co., Ltd.). The ΔyggS Δggt, ΔyggS Δggt ΔptsG, ΔyggS Δggt ΔmiaA, or ΔyggS Δggt ΔridA strain harboring pGshA*B was grown for 4 h by cultivation method B with 1 mM each Glu, 2-AB, and Gly. (B and C) The ATP concentrations in the culture medium (B) and the GlyA activity in the cell extract (C) were determined as described in Materials and Methods. Data are expressed as the means ± standard deviations of the data obtained after 3 repetitions of each assay.